### Dataset for "NanoBRET-based detection of ligand-receptor interactions at the neuropeptide FF receptor 1"



##### General information

* Authors: Hannah Lentschat and Annette G. Beck-Sickinger\* (\* = corresponding author)
* Affiliation: Institute of Biochemistry, Faculty of Life Sciences, Leipzig University, Leipzig, 04103 Germany
* Contact (e-mail): abeck-sickinger@uni-leipzig.de
* Date of collection (DD.MM.YYYY): 09.07.2021-09.04.2026
* Funding: German Research Foundation (DFG) through the SFB1423, project number 421152132, subproject B01 and under Germany´s Excellence Strategy – EXC-3105/1 – 533765739.





##### Data

The repository includes the following data types:

* Raw data from receptor activity and BRET binding measurements (.xlsx)
* GraphPad Prism files used to generate plots and figures (.prism)
* Structure adapted with PyMOL (.pse)
* Exported RP-HPLC chromatograms and mass spectra (.pdf)





##### Usage notes

The data can be accessed and viewed with the following software:

* .xlsx: Microsoft Excel or compatible spreadsheet software
* .prism: GraphPad Prism
* .pdf: any Standard pdf Viewer
* .pse: PyMOL





##### Data organization

Files are organized according to the figure numbers from the corresponding publication:

* Each folder corresponds to a figure and its subfigures
* Folders contain all raw data that was used in the production of the corresponding figure
* In the raw data, peptides are occasionally identified by their internal synthesis code (xn10 = NPVF; xw10.1 = Tam-NPVF; xw10.2 = Tam-Ahx2-NPVF; yd1 = Tam-TTDS-NPVF; vk8+9 = NPFF; xw11.1 = Tam-NPFF; xw11.2 = Tam-Ahx2-NPFF; zw3.2 = K(Tam)-NPFF; zw2 = SQA-NPFF; zw2.2 = K(Tam)-SQA-NPFF)





##### Methodological info

* All experimental procedures, data collection methods, and analytical protocols are described in detail in the "Experimental section" of the associated publication





##### License

This dataset is made available under the Creative Commons Attribution 4.0 International License (CC BY 4.0). Users are free to share and adapt the data, provided appropriate credit is given. When citing this dataset, please use:
Hannah Lentschat, Annette G. Beck-Sickinger (2026). Dataset for "NanoBRET-based detection of ligand-receptor interactions at the neuropeptide FF receptor 1". Open Access Repository and Archive (OPARA). Universität Leipzig. https://doi.org/10.25532/OPARA-1092

* URL in repository: https://opara.zih.tu-dresden.de/handle/123456789/2192
* Persistent identifier: DOI: https://doi.org/10.25532/OPARA-1163





##### Notes

* Some .pdf files referencing their own file path have been shortened for containing proprietary information. No original data has been modified or obfuscated





##### References

* EPFL (2022). README file for datasets - Best practices and template. Retrieved 03.11.2025 from https://infoscience.epfl.ch/server/api/core/bitstreams/e92aa9c8-c545-4d04-8b0b-8c6a54631fa5/content





##### Version and updates

* Version: 2.0
* Date of release: 10.04.2026
* Any subsequent updates or corrections will be listed in this section

  * Data were added upon Revision of the publication