The data are organized in folders with the same label of the images and plots of the paper. Some folders contain data for multiple figures of the paper, for example 1c_ED1c, contains data for Figure 1c and Figure ED1c. Raw data images and videos files are in .tif or Olympus format. The latter files are organized as follows: Filename.vsi _Filename_ (folder) > stack1 (subfolder) > > frame_t_0.ets To open this file drag the file frame_t_0.ets to Fiji/ImageJ or open it with Bio-Formats Importer. The scripts for the data analysis are in the following locations: - EB1 comet tracking and linear profiles generation: Figure1/1kl - EB1 radial profile generation: EDFigure2/ED2g - Invasion analysis time: Figure2/2d - Compartment area segmentation: Figure2/2eh - Speckle analysis: Figure3/3a4cdED9f/theta_Sperm - FRAP analysis:Figure3/3a4cdED9f/theta_HeLaCentrosome - Polymerization velocity analysis: Figure3/3a4cdED9f/vp_DrosoCentrosome_ED8f The scripts are in .ijm and .py format. .ijm scripts are Fiji/ImageJ scripts that can be dragged directly to Fiji/ImageJ (Version 2.14.0/1.54f used in this work). .py scripts are compiled in Python (version 3.9 in this work) running on PyCharm 2021.1.3 (in this work).The script "2-TrackEB1xTrackpyv7.py" for particle tracking is written with Jython Scripting and should be dragged and open in Fiji/ImageJ.