Schwabe, MaximilianIlker, EfeYang, Xingbo2026-06-192026-06-192026-06-122026-06-19https://opara.zih.tu-dresden.de/handle/123456789/2712https://doi.org/10.25532/OPARA-1471This dataset contains the data of subcellular distributions of the mitochondrial electron transport chain (ETC) flux within mouse oocytes inferred from the fluorescence lifetime imaging (FLIM) of NADH. The ETC flux distribution is obtained as a function of distance to the center of the oocyte, displaying a spatial gradient. This dataset includes the different responses of this subcellular ETC flux gradients to oxygen drop and temperature sweep perturbations of the oocytes. It contains the raw data of the ETC flux gradients across these perturbations as well as analysis of the origin of this gradient using reaction-diffusion and spatial kinetic modeling. The code used to generate and make plots from the data is available at https://github.com/MaxScw/mitoFluxGradientsAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/2::21::201::201-02