Each compressed file (.zip file) name corresponds to the measurement sample (the variant name, and if present, the chaperone name).
After uncompressing the archives, various folders are generated:
- the "Raw data files" folder contains the acquired photon stream files in .ht3 format.
- For all the FRET measurements presented in the manuscript, a folder named "BIN files - IPT-0p05-0p05-N-40" is also provided. It contains the analysed burst files with a maximum interphoton time of 0.05 ms, minimum interphoton noise of 0.05 ms, and a minimum total number of 40 photons. All the other settings used for analysis are mentioned in the "Settings.txt"*. 
- In the folders of OmpX 1 149 measurements without chaperones i.e. "xxDeg_0nM_Skp_Trimer" or "xxDeg_0nM_SurA", the "for RASP analysis" folder contains the pre-processed file used for RASP analysis.
- In the folders with OmpX+Chaperone measurements, the "FRET_OmpX_Skp_xnM" or "FRET_OmpX_SurA_xnM" are the files used for performing global chi-square minimization routine to obtain the thermodynamic parameters.

All measurements were done in a buffer containing  20 mM TrisHCl and 150 mM NaCl, pH 8.0, unless otherwise stated.

*Each column, in the order as present in the "Settings.txt" file, stands for:
- Brd_RR(1) ... lower border of the microtime window for red excitation red emission
- Brd_RR(2) ... upper border of the microtime window for red excitation red emission
- Brd_GGR(1) ... lower border of the microtime window for green excitation green/red emission
- Brd_GGR(2) ... upper border of the microtime window for green excitation green/red emission
- threIT ... minimal threshold for inter-photon time burst identification
- threITN ... maximal threshold for inter-photon time noise region identification
- minPhs ... minimal number of consecutive photons satisfying threIT for burst identification
- setLeeFilter ... Lee-filter number of photons
- boolTotal ... boolean -> 1: merged photons from acceptor and donor excitation, 0: separated thresholds minGR and minR0 for donor and acceptor excitation
- minGR ... minimal photon threshold from donor excitation
- minR0 ... minimal photon threshold from acceptor excitation
- boolFLA ... boolean -> 1: fast lifetime analysis (average mean microtime - average microtime IRF); 0: individual decay fits for each burst
- gGG ... correction factor for anisotropy calculation of donor fluorophore
- gRR ... correction factor for anisotropy calculation of acceptor fluorophore
- boolPostA ... boolean option 1: storage of photon data in PData files
- corrIRF_G ... average microtime channel of the green IRF
- corrIRF_R ... average microtime channel of the red IRF