https://opara.zih.tu-dresden.de/xmlui/handle/123456789/6054 Blood is the most encountered type of biological evidence in violent crimes and contains pertinent information to a forensic investigation. The false presumption that blood encountered at a crime scene is human may not be realised until after costly and sample-consuming tests are performed. To address the question of blood origin, the novel application of visible-near infrared hyperspectral imaging (HSI) is used for the detection and discrimination of human and animal bloodstains. The HSI system used is a portable, non-contact, non-destructive method for the determination of blood origin. A support vector machine (SVM) binary classifier was trained for the discrimination of bloodstains of human (n = 20) and five animal species: pig (n = 20), mouse (n = 16), rat (n = 5), rabbit (n = 5), and cow (n = 20). On an independent test set, the SVM model achieved accuracy, precision, sensitivity, and specificity values of 96, 97, 95, and 96%, respectively. Segmented images of bloodstains aged over a period of two months were produced, allowing for the clear visualisation of the discrimination of human and animal bloodstains. The inclusion of such a system in a forensic investigation workflow not only removes ambiguity surrounding blood origin, but can potentially be used in tandem with HSI bloodstain age determination methods for rapid on-scene forensic analysis. 2026-04-08T05:57:10Z https://opara.zih.tu-dresden.de/xmlui/handle/123456789/6117 Discrimination of human and animal bloodstains using hyperspectral imaging Babian, Carsten; Cooney, Gary Sean; Chalopin, Claire; Köhler, Hannes To address the question of blood origin, the novel application of visible-near infrared hyperspectral imaging (HSI) is used for the detection and discrimination of human and animal bloodstains. The HSI system used is a portable, non-contact, non-destructive method for the determination of blood origin. A support vector machine (SVM) binary classifier was trained for the discrimination of bloodstains of human (n = 20) and five animal species: pig (n = 20), mouse (n = 16), rat (n = 5), rabbit (n = 5), and cow (n = 20). Data and methods are described in detail in the attached documents and in https://doi.org/10.1007/s12024-023-00689-0. 2024-01-01T00:00:00Z