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Datasets of male meiotic spindles. 1-Metaphase 2-Anaphase onset 3-Anaphase 1 4-Anaphase 2 5-Anaphase 3 6-Anaphase 4
A new method of surfactant administration, combining advantages of INSURE and LISA/MIST was developed at the TU Dresden. Retrospective patient data is analyzed to evaluate this approach.
This dataset contains the X-ray micro-computed tomography (µCT) volumes of the examined teeth as a 3D reference for all subsequent examinations. The µCT volumes were acquired on the intact, non-embedded teeth using a µCT specimen scanner (SCANCO vivaCT 40, SCANCO Medical AG, Brüttisellen, Switzerland) at the Bone Lab Dresden. All µCT scans were recorded with the following parameters: voltage: 70 kV, exposure time: 0.35 s, current: 0.114 mAs. The voxel size is 10.5 µm x 10.5 µm x 10.5 µm.
This dataset contains the images of 24 selected thin sections examined with polarized light microscopy. The thin sections were examined with a Leica DMRB polarizing microscope (Leica Microsystems GmbH, Wetzlar, Germany) using a magnification of 2.5 times. The photographs during examination were acquired with a digital camera (sony α7, Sony, Tokyo, Japan) using the following settings: exposure time of 1/160 s, ISO sensitivity of 125. Three photographs are available for each thin section. The first photograph shows the examination using conventional polarized light microscopy with the polarizer and analyzer rotated at 90° to each other (cross-polarized PLM, or PLMc). The second photograph displays the thin section using polarized light microscopy as in PLMc with an additional λ-plate prior to the analyzer (red-plate PLM, or PLMr). The third photograph shows the examination with transmission light microscopy without the analyzer being used (bright-field microscopy, or PLMb). The order of the images in the folders is always the same: PLMc, PLMr, PLMb, where for each photo always a processed .JPG file and the raw data as acquired .ARW file is available. The pixel size in each image is 2.28 µm x 2.28 µm.
This dataset contains the raw sequences and images of 24 selected thin sections cut from 17 tooth samples, which were examined with advanced polarized light microscopy. Using a polarization camera with a 2x2 pattern of polarizers on the sensor and varying linearly polarized illumination states, depolarized light microscopy images were created by evaluating the degree of polarization (DOP) within thin sections. To generate the data, the thin sections were examined using a modified Leica DMRB microscope (Leica Microsystems GmbH, Wetzlar, Germany) with the complementary implementation of a FLIR polarization camera (FLIR Integrated Imaging Solutions Inc., Ludwigsburg, Germany). The pixel size of the processed images is 3.93 µm x 3.93 µm. Since multiple areas of interests were examined for several thin sections, 29 data sets are available. For each data set, the sequence of raw images of the polarization camera as well as processed DLM images including representations of the DOP and of the intensity are available. Note, that the DLM images must be flipped horizontally compared to the conventional polarized light microscopy images acquired with the same microscope.