Research Data Repository of Saxon Universities
OPARA is the Open Access Repository and Archive for Research Data of Saxon Universities.
Researchers of Saxon Universities can either publish their research data on OPARA, or archive it here to comply with requirements of funding acencies and good scientic practice, without public access.
You can find the documentation of this service at the ZIH Data Compendium websites. If you need suppourt using OPARA please contact the Servicedesk of TU Dresden.
Please note: The OPARA service was recently upgraded to a new technical platform (this site). Previously stored data will not be available here immediately. It can be found at the still active old version of OPARA. These stock data will be migrated in near future and then the old version of OPARA will finally be shut down. Existing DOIs for data publications remain valid.
Communities in OPARA
Select a community to browse its collections.
Recent Submissions
Evolution and adaptations of the seminal proteome in an insect with traumatic insemination
(Technische Universität Dresden, 2026-04-14) Garlovsky, Martin
The protein composition of sperm and seminal fluid are key to male fitness. However, we currently lack an understanding of the factors that shape seminal proteome composition. The common bedbug (Cimex lectularius) mates by traumatic insemination, subjecting the ejaculate to a unique selective environment as sperm traverse the female genital and paragenital system. We provide the first high-throughput proteomic characterisation of the sperm and seminal fluid proteome in a hemimetabolous insect and the first in-depth proteomic characterisation of the male bedbug reproductive system. Our analysis revealed conserved and unique features of the sperm and seminal fluid proteome with possible links to features of sperm behaviour linked to traumatic insemination. The sperm proteome showed elevated rates of molecular evolution, unlike most other studied species. Conversely, the sperm proteome also contained many conserved proteins. Notably, we found an expansion of Sperm-leucylaminopeptidases (S-Laps) in bedbugs and other hemimetabolous insects, suggesting the origin of S-Laps is perhaps even more ancient than previously thought. Using in silico protein-ligand binding predictions, we show that S-Laps have likely retained catalytic activity. Our results provide a list of candidate proteins involved in reproduction and a useful resource for future studies of this expanding global pest.
Bicycle Driving Behaviour in Germany: A Driving Parameter Dataset Across 100 Cities based on CITY CYCLING GPS trajectories
(Technische Universität Dresden, 2026-04-14) Lissner, Sven; Lindemann, Paul; Huber, Stefan
The dataset presented was created as part of the cycling campaign City Cycling in Germany during the years 2022–2024. The campaign involved more than 3,000 municipalities across the entire country. Over a three-week period, which participants could freely select between May 1 and September 30, the goal was to replace as many car trips as possible with bicycle trips. Participants could use a smartphone application to record their data. The recorded GPS trajectories were transferred from the smartphone to a data lake as a database dump and were then regularly retrieved by a backend server for data pre-processing. During this process, trips, activities, modes of transport, and driving modes were identified. For the dataset presented, individual trajectories were processed with respect to several core variables characterizing cycling behaviour. The dataset was spatially filtered beforehand. A total of 109 cities and municipalities were selected based on variables such as population size, the number of campaign participants, topography, the modal share of cycling, and geographic location within Germany, in order to obtain a representative impression of cycling behaviour. As a result, more than 8 million trip records with 37 distinct variables were collected over the data years 2022–2024.The dataset was created and analysed within the scope of the DFG funded project "Bicycle Driving Behaviour in Germany", Project number 514587991
Dataset for "NanoBRET-based detection of ligand-receptor interactions at the neuropeptide FF receptor 1" V2
(Universität Leipzig, 2026-04-14) Lentschat, Hannah; Beck-Sickinger, Annette G.
The neuropeptide FF receptor 1 (NPFFR1) belongs to the RF-amide G protein-coupled receptor family. Even though it is a promising therapeutic target for the treatment of chronic pain, this receptor still has not been used as a drug target. A detailed understanding of its ligand-binding and activation mechanisms is essential for the rational design of novel modulators. In this study, we developed a non-radioactive, nanoBRET-based ligand-binding assay to investigate ligand interactions of neuropeptide FF (NPFF) and neuropeptide VF (NPVF) with the NPFFR1. Fluorescently labeled NPFF and NPVF analogs were synthesized by conjugating a 6-carboxytetramethylrhodamine fluorophore at distinct positions, while a nanoluciferase was fused to the N-terminus of the NPFFR1 to serve as the BRET donor. This approach enables quantitative measurement of ligand binding and provides insights into the relative orientation of the ligand and receptor. Distinct BRET signal profiles for NPFF and NPVF, with a smaller window for NPVF compared to NPFF when directly labeled with the fluorophore, indicate differences in the binding orientation. Furthermore, deletion of the N-terminal residues of the receptor revealed that this region is dispensable for ligand recognition and binding in the NPFFR1. The assay was confirmed for small molecule NPFFR1 ligands, such as hederagenin, offers new opportunities to explore subtype selectivity and will guide drug discovery targeting the RF-amide receptor family.
Rapid quantification method of pharmaceuticals in urban wastewater using UHPLC-Orbitrap mass spectrometry – a multi target approach for EU-directive advanced treatment evaluation
(Technische Universität Dresden, 2026-04-13) Hofmann, Uta
Pharmaceutical residues have become contaminants of emerging concern in wastewater, as inadequate removal by wastewater treatment plants can result in ecotoxicological impacts on aquatic ecosystems. For this reason, the EU Urban Wastewater Treatment Directive (UWWTD 2024/3019) requires the implementation of quaternary treatment upgrades and the regular monitoring of their effectiveness in European Wastewater Treatment Plants (WWTPs). In this study, a sensitive, reliable, and cost-effective analytical method was developed and validated for the simultaneous determination of twelve indicator substances, including nine compounds proposed by the UWWTD, and three substitutes in various influent and effluent of WWTPs. The method is based on solid-phase extraction (SPE) of 2-mL-wastewater samples using, followed by reversed-phase liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) employing an Orbitrap Exploris™ 240. Detection was carried out using heated electrospray ionization in positive ionization mode (HESI+), except diclofenac (HESI-). Quantification was performed using SIM scans, while high-resolution full-scan spectra were acquired for confirmation. Limits of Quantification (LOQ) ranged mostly between 15 and 30 ng/L, except for candesartan, irbesartan and diclofenac with LOQ > 50 ng/L. A laboratory validation demonstrated high precision and accuracy for all selected pharmaceuticals. Further, the method was successfully applied to raw and treated wastewater from WWTPs of different size classes in the border region of Saxony (Germany) and Northern Bohemia (Czech Republic). This method therefore offers a rapid quantification approach for assessing the removal efficiency of pharmaceuticals and potential substitutes categorized in the UWWTD.
Data corresponding to publication: "Classical spin liquids from frustrated Ising models in hyperbolic space" by F. Köhler et al (2026)
(Technische Universität Dresden, 2026-04-10) Vojta, Matthias
This dataset contains the data and scripts corresponding to the figures in the publication F. Köhler, J. Erdmenger, R. Moessner, M. Vojta, "Classical spin liquids from frustrated Ising models in hyperbolic space", Phys. Rev. E (2026).