Polarized light microscopy (PLM) images of occlusal caries lesions

Abstract

This dataset contains the images of 24 selected thin sections examined with polarized light microscopy. The thin sections were examined with a Leica DMRB polarizing microscope (Leica Microsystems GmbH, Wetzlar, Germany) using a magnification of 2.5 times. The photographs during examination were acquired with a digital camera (sony α7, Sony, Tokyo, Japan) using the following settings: exposure time of 1/160 s, ISO sensitivity of 125. Three photographs are available for each thin section. The first photograph shows the examination using conventional polarized light microscopy with the polarizer and analyzer rotated at 90° to each other (cross-polarized PLM, or PLMc). The second photograph displays the thin section using polarized light microscopy as in PLMc with an additional λ-plate prior to the analyzer (red-plate PLM, or PLMr). The third photograph shows the examination with transmission light microscopy without the analyzer being used (bright-field microscopy, or PLMb). The order of the images in the folders is always the same: PLMc, PLMr, PLMb, where for each photo always a processed .JPG file and the raw data as acquired .ARW file is available. The pixel size in each image is 2.28 µm x 2.28 µm.