Subcellular ETC flux data in mouse oocytes under oxygen drop and temperature sweep experiments
Type of the data | Dataset | |
Total size of the dataset | 26377389734 | |
Author | Schwabe, Maximilian | |
Author | Ilker, Efe | |
Author | Yang, Xingbo | |
Upload date | 2026-06-19T12:29:55Z | |
Publication date | 2026-06-19T12:29:55Z | |
Data of data creation | 2026-06-12 | |
Publication date | 2026-06-19 | |
Abstract of the dataset | This dataset contains the data of subcellular distributions of the mitochondrial electron transport chain (ETC) flux within mouse oocytes inferred from the fluorescence lifetime imaging (FLIM) of NADH. The ETC flux distribution is obtained as a function of distance to the center of the oocyte, displaying a spatial gradient. This dataset includes the different responses of this subcellular ETC flux gradients to oxygen drop and temperature sweep perturbations of the oocytes. It contains the raw data of the ETC flux gradients across these perturbations as well as analysis of the origin of this gradient using reaction-diffusion and spatial kinetic modeling. The code used to generate and make plots from the data is available at https://github.com/MaxScw/mitoFluxGradients | |
Public reference to this page | https://opara.zih.tu-dresden.de/handle/123456789/2712 | |
Public reference to this page | https://doi.org/10.25532/OPARA-1471 | |
Publisher | Technische Universität Dresden | |
Licence | Attribution-NonCommercial-NoDerivatives 4.0 International | en |
URI of the licence text | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
Specification of the discipline(s) | 2::21::201::201-02 | |
Title of the dataset | Subcellular ETC flux data in mouse oocytes under oxygen drop and temperature sweep experiments | |
Research instruments | Two-photon scanning confocal microscope with Becker and Hickle Time Correlated Single Photon Counting (TCSPC) acquisition system with HPM-100-40 GaAsP hybrid PMT detector and a 80MHz pulsed MaiTai DeepSee Ti:Sapphire laser (Spectra-Physics). | |
Underlying research object | Mouse oocytes | |
Software | MATLAB R2023a | |
Project abstract | Mitochondria are metabolic hubs of the cell that provide energy and metabolites to meet the energetic, biosynthetic and signaling demands of the cell. Mitochondrial activities are characterized by the metabolic fluxes through its internal metabolic pathways. One of the most important mitochondrial metabolic pathways is the electron transport chain (ETC), where electron carriers such as NADH donates its electrons to oxygen to power mitochondrial respiration. Mitochondrial activities are dynamically and spatially regulated during organism development to ensure robust development. Recent work has revealed the existence of a subcellular ETC flux gradient within a single mouse oocyte, where mitochondria closer to the cell membrane display a higher ETC flux, but the mechanism underlying the formation of this gradient is unknown. In this work, we study the origin of the ETC flux gradients by modulating the gradients through perturbations of external oxygen concentration and temperature. Interpreting the data with spatial kinetic modeling of mitochondrial respiration, we discover that the subcellular ETC flux gradient cannot be explained by reaction-diffusion of oxygen alone, but is a result of mitochondrial heterogeneity where mitochondria closer to the cell membrane display larger ETC flux capacity and lower oxygen sensitivity. Our work suggests that kinetically distinct subpopulations of mitochondria are spatially sorted according to their metabolic activities to form intracellular metabolic gradients. | |
Funding Acknowledgement | This study received support from the Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) under Germany’s Excellence Strategy — EXC-2068-390729961 — Cluster of Excellence Physics of Life of TU Dresden and from the French government under the France 2030 investment plan, as part of the Initiative d’Excellence d’Aix-Marseille Université-Amidex (AMX-23-CEI-064). | |
Project title | Heterogeneous flux capacity and oxygen sensitivity lead to subcellular ETC flux gradients in mouse oocytes |
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