Data MembraneTension HeLa in mitosis

Fischer-Friedrich, Elisabeth; Panda, Yogishree Arabinda

Data MembraneTension HeLa in mitosis

Contributing person datacite.contributor.DataCollector	Yogishree Arabinda Panda
Description of the data datacite.resourceType	1) FliptR FLIM measurements recorded with a Zeiss LSM780 confocal (lsm files) in combination with a Becker and Hickl Flim scope (sdt files) We used this to generate the figures 1, 2, 3
Type of the data datacite.resourceTypeGeneral	Collection
Total size of the dataset datacite.size	89021375068
Author dc.contributor.author	Fischer-Friedrich, Elisabeth
Author dc.contributor.author	Panda, Yogishree Arabinda
Upload date dc.date.accessioned	2025-02-11T10:19:01Z
Publication date dc.date.available	2025-02-11T10:19:01Z
Publication date dc.date.issued	2025-02-11
Abstract of the dataset dc.description.abstract	In this project, we were investigating the membrane tension in mitotically arrested HeLa cells using two different methods: the optical membrane tension sensor FliptR and membrane tether forces. FliptR is a fluorescent dye that localizes to the plasma membrane. Perfoming FLIM on the plasma membrane fluorescence signal, we fitted fluorescence lifetimes as a measure of membrane tension. Further, coated tips of an pyramidal AFM cantilever (Bruker MLCT) were used to pull membrane tethers. The full description of the Methods is in the uploaded manuscript draft "PandaEtAl.pdf" contained in PaperArabinda_05Feb2025.zip The microscopy/AFM data used for analysis and generation of the figures in this manuscript draft are uploaded in this archive. Figure numbers in the naming of the folders are corresponding to the figure numbers in this manuscript draft and not to figure numbers in a potentially updated later version of the manuscript.
Public reference to this page dc.identifier.uri	https://opara.zih.tu-dresden.de/handle/123456789/1273
Public reference to this page dc.identifier.uri	https://doi.org/10.25532/OPARA-742
Publisher dc.publisher	Technische Universität Dresden
Licence dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 International	en
URI of the licence text dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
Specification of the discipline(s) dc.subject.classification	3
Title of the dataset dc.title	Data MembraneTension HeLa in mitosis
Research instruments opara.descriptionInstrument	Zeiss LSM780 with Becker and Hickl FLIM at CRTD, ground floor, CMCB facility: FlipTR FLIM (additional delay cable): output: sdt files, lsm files
Research instruments opara.descriptionInstrument	Zeiss LSM700+Bruker Nanowizard 4XP AFM, PoL facility: membrane tether pulling experiments
Research instruments opara.descriptionInstrument	Zeiss LSM510+JPK Nanowizard 1, Fischer-Friedrich Lab: cortical tension measurements, .out files
Research instruments opara.descriptionInstrument	Zeiss LSM780 confocal, CMCB facility, CRTD ground floor: output: lsm files showing Lifeact-mScarlet fluorscence in HeLa cells, filopodia imaging
Research instruments opara.descriptionInstrument	Zeiss LSM780 with Becker and Hickl FLIM at CRTD, ground floor, AFM Nanowizard 1, CMCB facility: FlipTR FLIM (additional delay cable): output: sdt files, lsm files, .out files for AFM
Underlying research object opara.descriptionObject.Other	HeLa cell culture
Software opara.descriptionSoftware.ResourceProduction	Zen, Becker & Hickl software
Project abstract opara.project.description	The plasma membrane and its associated proteins form a critical signaling hub, mediating communication between the extracellular environment and the intracellular space. Previous research suggests that both membrane trafficking and signaling activity are influenced by mechanical tension in the plasma membrane. Despite its importance, the mechanisms by which cells regulate membrane tension remain poorly understood. Using the optical tension sensor FliptR and AFM-assisted tether force measurements, we investigate plasma membrane tension regulation in mitotic cells by measuring tension changes following cytoskeletal and cell shape perturbations. Our findings show that in both assays, reported tensions are critically influenced by the cytoskeleton, with distinct trends under different perturbations, highlighting the conceptual differences between bare and apparent membrane tension. By integrating experimental data with theoretical modeling, we demonstrate that the actin cytoskeleton regulates bare membrane tension through two distinct mechanisms: (i) modulation of intracellular hydrostatic pressure and (ii) adjustment of polymerization forces in actin-rich finger-like protrusions.
Project title opara.project.title	Regulation of plasma membrane tension through hydrostatic pressure and actin protrusion forces