Supplementary data for the publication "Cell size reduction distinctly scales spindle elongation and chromosome segregation in C. elegans"
Countries to which the data refer | GERMANY | |
Description of the data | Lattice light-sheet fluorescence microscopy (LLSM) datasets of different C. elegans embryonic conditions (wild type, C27D9.1(RNAi), ani-2(RNAi)) for the study of spindle length scaling and chromosome segregation dynamics. Each LLSM dataset is a six-dimensional dataset (x, y, z, time, 2 channels) acquired with a 10.3 s time interval between frames and a pixel size of 100 nm (xy) and 500 nm (z). The LLSM datasets were acquired from the earliest embryonic cell stage to the late embryonic stage. | |
Type of the data | Dataset | |
Type of the data | Image | |
Total size of the dataset | 845513308277 | |
Author | Okafornta, Chukwuebuka William | |
Upload date | 2026-06-29T15:06:19Z | |
Publication date | 2026-06-29T15:06:19Z | |
Data of data creation | 2026-09-29 | |
Publication date | 2026-06-29 | |
Abstract of the dataset | How embryos adapt their internal cellular machinery to reductions in cell size during development remains a fundamental question in cell biology. Here, we use high-resolution lattice light-sheet fluorescence microscopy and automated image analysis to quantify lineage-resolved mitotic spindle and chromosome segregation dynamics from the 2- to 64-cell stages in Caenorhabditis elegans embryos. While spindle length scales with cell size across both wild-type and size-perturbed embryos, chromosome segregation dynamics remain largely invariant, suggesting that distinct mechanisms govern these mitotic processes. Combining femtosecond laser ablation with large-scale electron tomography, we find that mid-spindle microtubules mediate chromosome segregation dynamics and remain uncoupled from cell size across all stages of early development. In contrast, spindle elongation is driven by cortically anchored motor proteins and astral microtubules, rendering it sensitive to cell size. Incorporating these experimental results into an extended stoichiometric model for both the spindle and chromosomes, we find that allowing only cell size and microtubule catastrophe rates to vary reproduces spindle pole-to-pole dynamics across development. The same model also accounts for centrosome separation and pronuclear positioning in the one-cell C. elegans embryo, spindle-length scaling across nematode species spanning ~100 million years of divergence, and spindle rotation in human cells. Thus, a unified stoichiometric framework provides a predictive, mechanistic account of spindle and nuclear dynamics across scales and species. | |
Public reference to this page | https://opara.zih.tu-dresden.de/handle/123456789/2780 | |
Public reference to this page | https://doi.org/10.25532/OPARA-1498 | |
Publisher | Technische Universität Dresden | |
Licence | Attribution-ShareAlike 4.0 International | en |
URI of the licence text | http://creativecommons.org/licenses/by-sa/4.0/ | |
Specification of the discipline(s) | 2::21::201::201-03 | |
Specification of the discipline(s) | 2::21::201::201-06 | |
Title of the dataset | Supplementary data for the publication "Cell size reduction distinctly scales spindle elongation and chromosome segregation in C. elegans" | |
Research instruments | Lattice Lightsheet Microscope (custom-built), Advanced Imaging Facility, Max-Planck-Institut für Molekulare Zellbiologie und Genetik (MPI-CBG) | |
Underlying research object | C. elegans embryo | |
Funding Acknowledgement | Deutsche Forschungsgemeinschaft, DFG, grant number: 258577783 Simons Foundation CCBx program (SF, grant number 1157392) |
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