HeLa metaphase spindle tomographic data sets and analysis

Kiewisz, Robert

HeLa metaphase spindle tomographic data sets and analysis

datacite.FundingReference.funderName datacite.FundingReference.funderName	Europäische Union
Contributing person datacite.contributor.ProjectLeader	Müller-Reichert, Thomas (orcid: 0000-0003-0203-1436)
Contributing person datacite.contributor.other	Robert Kiewisz
datacite.description.TableOfContents datacite.description.TableOfContents	Metaphase_#1 ├── Analysis_ASGA_v0.35 # Excel sheets containing all analyzed results obtained from ASGA v0.35 ├── MT_Models # 3D spatial graph models of MTs in HeLa #1 │ └── T_0475_meta_7A-7C_spatialgraph_All_MTs.am │ └── T_0475_meta_7A-7C_spatialgraph_ASGA_Analysis.am │ └── T_0475_meta_7A-7C_spatialgraph_KMTs.am │ └── T_0494_meta_10B-10E_spatialgraph_ASGA_Analysed.am ├── Surfaces # 3D models obtained from tomographic volume segmentation with Zib Amira │ └── T_0475_meta_7A-7C_Centrosome.am │ └── T_0475_meta_7A-7C_Chromosome.am └── Tomogram_Stack # Tomographic volume saved in binary .am format Metaphase_#2 ├── Analysis_ASGA_v0.35 # Excel sheets containing all analyzed results obtained from ASGA v0.35 ├── MT_Models # 3D spatial graph models of MTs in HeLa #2 │ └── T_0479_meta_15A-15C_spatialgraph_All_MTs.am │ └── T_0479_meta_15A-15C_spatialgraph_ASGA_Analysis.am │ └── T_0479_meta_15A-15C_spatialgraph_KMTs.am │ └── T_0479_meta_15A-15C_spatialgraph_ASGA_Analysed.am ├── Surfaces # 3D models obtained from tomographic volume segmentation with Zib Amira │ └── T_0479_meta_15A-15C_Centrosome.am │ └── T_0479_meta_15A-15C_Chromosome.am └──Tomogram_Stack # Tomographic volume saved in binary .am format Metaphase_#3 ├── Analysis_ASGA_v0.35 # Excel sheets containing all analyzed results obtained from ASGA v0.35 ├── MT_Models # 3D spatial graph models of MTs in HeLa #3 │ └── T_0494_meta_10B-10E_spatialgraph_All_MTs.am │ └── T_0494_meta_10B-10E_spatialgraph_ASGA_Analysis.am │ └── T_0494_meta_10B-10E_spatialgraph_KMTs.am │ └── T_0494_meta_10B-10E_spatialgraph_ASGA_Analysed.am ├── Surfaces # 3D models obtained from tomographic volume segmentation with Zib Amira │ └── T_0494_meta_10B-10E_Centrosome.am │ └── T_0494_meta_10B-10E_Chromosome.am └──Tomogram_Stack # Tomographic volume saved in binary .am format
Documentation of the data datacite.description.TechnicalInfo	Data Processing: Reconstruction in IMOD, Modeling in ZIB Amira, Analysis in ASGA v0.35 Methods: high-pressure freezing, freeze substitution, epond infiltration and embedding, serial sectioning, Electron tomography
Additional geographical or spatial references datacite.geolocation	Saxony
Countries to which the data refer datacite.geolocation.iso3166	GERMANY
Description of the data datacite.resourceType	Tomographic data sets and analyzed data 3D tomographic model of HeLa cell obtained with 300kV TEM
Type of the data datacite.resourceTypeGeneral	Model
Type of the data datacite.resourceTypeGeneral	Text
Total size of the dataset datacite.size	204526715255
Author dc.contributor.author	Kiewisz, Robert
Upload date dc.date.accessioned	2021-09-02T12:49:21Z
Publication date dc.date.available	2021-09-02T12:49:21Z
Publication date dc.date.available	2026-06-05T11:16:03Z
Data of data creation dc.date.created	2021
Publication date dc.date.issued	2021-09-02
Abstract of the dataset dc.description.abstract	This collection of datasets belongs to the eLife paper titled "Three-dimensional structure of kinetochore-fibers in human mitotic spindles": The collection includes all raw datasets of three HeLa Kyoto metaphase cells, that were acquired using electron tomography technique. This collection is also included all processed data as well as analysis as were shown in the publication.
Public reference to this page dc.identifier.uri	https://opara.zih.tu-dresden.de/handle/123456789/2526
Public reference to this page dc.identifier.uri	https://doi.org/10.25532/OPARA-128
dc.language dc.language	eng
Publisher dc.publisher	Technische Universität Dresden
Licence dc.rights	Attribution 4.0 International
URI of the licence text dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
Specification of the discipline(s) dc.subject.classification	2::21
Specification of the discipline(s) dc.subject.classification	2
Title of the dataset dc.title	HeLa metaphase spindle tomographic data sets and analysis
Research instruments opara.descriptionInstrument	Leica AFS II
Research instruments opara.descriptionInstrument	Wohlwend HPF compact 03
Research instruments opara.descriptionInstrument	Leica ultramicrotome UC5
Research instruments opara.descriptionInstrument	FEI F30
Software opara.descriptionSoftware.ResourceProduction	IMOD (Version 4.12.8)
Software opara.descriptionSoftware.ResourceProduction	Zib Amira (Version 2021.05)
Software opara.descriptionSoftware.ResourceProduction	ASGA (Version 0.35)