DNA ruler measurement for accuracy and precision of multiwell plate smFRET experiments

Schlierf, Michael

DNA ruler measurement for accuracy and precision of multiwell plate smFRET experiments

Contributing person datacite.contributor.ProjectLeader	Schlierf, Michael (orcid: 0000-0002-6209-2364)
Contributing person datacite.contributor.RightsHolder	Schlierf Lab
Contributing person datacite.contributor.WorkPackageLeader	Hartmann, Andreas
Documentation of the data datacite.description.TechnicalInfo	Data Acquisition: smFRET experiments Methods: time-correlated single photon counting for single-molecule FRET spectroscopy Resource Type: Photon streams of 96-well (A01 to H12) smFRET data on dsDNA with donor-acceptor pairs at 9 and 21 bp distance. Instrument response functions (IRF) of the data set. software package for data curation, data analysis, and data visualisation can be found here: https://github.com/SchlierfLAB/autoFRET
References to related material datacite.relatedItem.References	https://github.com/SchlierfLAB/autoFRET
Description of the data datacite.resourceType	smFRET data for multiwell plate measurements (smFRET_multi) raw (ht3) and pre-analysed (bin) data of smFRET measurements for high-content, multiwell plate smFRET experiments.
Type of the data datacite.resourceTypeGeneral	Dataset
Total size of the dataset datacite.size	1675009776
Author dc.contributor.author	Schlierf, Michael
Upload date dc.date.accessioned	2023-03-17T13:01:39Z
Publication date dc.date.available	2023-03-17T13:01:39Z
Publication date dc.date.available	2026-06-08T12:25:05Z
Data of data creation dc.date.created	2021-2023
Publication date dc.date.issued	2023-03-17
Abstract of the dataset dc.description.abstract	HT3 data and analysed *.bin files for 96 measurement repeats A01 to H12 in a 96-well plate, multiparameter detection smFRET experiment. Data can be analysed and visualised using autoFRET package at https://github.com/SchlierfLAB/autoFRET
Public reference to this page dc.identifier.uri	https://opara.zih.tu-dresden.de/handle/123456789/2586
Public reference to this page dc.identifier.uri	https://doi.org/10.25532/OPARA-202
Publisher dc.publisher	Technische Universität Dresden
Licence dc.rights	Attribution-NonCommercial 4.0 International
URI of the licence text dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0/
Specification of the discipline(s) dc.subject.classification	3::32
Specification of the discipline(s) dc.subject.classification	2::21::201::201-01
Title of the dataset dc.title	DNA ruler measurement for accuracy and precision of multiwell plate smFRET experiments
Research instruments opara.descriptionInstrument	custom-built multiwell plate, multiparameter smFRET microscope
Underlying research object opara.descriptionObject.Other	dsDNA
Software opara.descriptionSoftware.Other	autoFRET
Project title opara.project.title	Multi-well smFRET (Multi_smFRET)

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