Next Generation Sequencing Data
Type of the data | Dataset | |
Total size of the dataset | 7548002241 | |
Author | Thielecke, Lars | |
Upload date | 2024-09-16T07:58:44Z | |
Publication date | 2024-09-16T07:58:44Z | |
Publication date | 2024-09-16 | |
Abstract of the dataset | Raw and randomly sampled NGS data of all 43 mice, including the corresponding raw and error-corrected barcode lists. | |
Public reference to this page | https://opara.zih.tu-dresden.de/handle/123456789/940 | |
Public reference to this page | https://doi.org/10.25532/OPARA-616 | |
Publisher | Technische Universität Dresden | |
Licence | Attribution-NonCommercial-NoDerivatives 4.0 International | en |
URI of the licence text | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
Specification of the discipline(s) | 2::21::201::201-03 | |
Title of the dataset | Next Generation Sequencing Data | |
Project abstract | The sustained production of blood and immune cells is driven by a pool of hematopoietic stem and progenitor cells (HSPCs). Due to the intrinsic heterogeneity of HSPCs, the composition of emergent clones changes over time, leading to reduced clonality in aging mice, with similar trends in humans. Theoretical analyses suggest that clonal conversion rates and clonality levels depend not only on HSPC heterogeneity, but also on stress conditions and overall hematopoietic turnover. Stem cell transplantations remain the only curative option for many hematologic diseases, increasingly considered viable for elderly individuals. However, age-related changes in clonality post-transplantation, particularly concerning HSC niches of different ages, are not well-defined. We conducted an annotated barcode-based assessment of clonality to investigate post-transplantation changes in both homo- and hetero-chronic settings, combined with low- and high-intensity pre-conditioned recipients. Robust and polyclonal engraftment was observed across all groups, with no substantial differences in the number of unique barcodes between young and old HSCs. In particular, aged HSPCs transplanted into severely pre-conditioned hosts did not significantly alter HPC clonality, nor did the age of the recipient. However, reduced pre-conditioning, while allowing full lymphoid reconstitution, revealed age-related differences in hematopoietic clonality. Myeloid lineage bias, a hallmark of aged HSCs, was confirmed at the clonal level. Overall, aged HSCs maintained clonal diversity similar to young HSCs under stress, while low-intensity conditioning influenced clonal dynamics. These findings challenge current paradigms and underscore the complex interactions between aging, transplantation conditions, and clonal dynamics, necessitating further investigation. | |
Project title | Publication - Clonality in post-transplantation murine hematopoiesis is unaffected by aging |
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