Measuring active fluctuations of the cortex in mitosis by embedding an AFM cantilever pyrimidal tip

Narinder, Narinder; Elisabeth, Fischer-Friedrich

Measuring active fluctuations of the cortex in mitosis by embedding an AFM cantilever pyrimidal tip

Type of the data datacite.resourceTypeGeneral	Collection
Total size of the dataset datacite.size	1900730519
Author dc.contributor.author	Narinder, Narinder
Author dc.contributor.author	Elisabeth, Fischer-Friedrich
Upload date dc.date.accessioned	2025-11-04T09:20:07Z
Publication date dc.date.available	2025-11-04T09:20:07Z
Data of data creation dc.date.created	2025-10-28
Publication date dc.date.issued	2025-11-04
Abstract of the dataset dc.description.abstract	Living cells exhibit non-equilibrium dynamics emergent from the intricate interplay between molecular motor activity and its viscoelastic cytoskeletal matrix. The deviation from thermal equilibrium can be quantified through frequency-dependent effective temperature or time-reversal symmetry breaking quantified e.g. through the Kullback-Leibler divergence. Here, we investigate the fluctuations of an AFM tip embedded within the active cortex of mitotic human cells with and without perturbations that reduce cortex activity through inhibition of material turnover or motor proteins. While inhibition of motor activity significantly reduces both effective temperature and time irreversibility, inhibited material turnover leaves the effective temperature largely unchanged but lowers the time irreversibility and entropy production rate. Our experimental findings in combination with a minimal model highlight that time irreversibility, effective temperature and entropy production rate can follow opposite trends in active living systems, challenging in particular the validity of effective temperature as a proxy for the distance from thermal equilibrium. Furthermore, we propose that the strength of thermal noise and the occurrence of time-asymmetric deflection spikes in the dynamics of regulated observables are inherently coupled in living systems, revealing a previously unrecognized link between entropy production and time irreversibility.
Public reference to this page dc.identifier.uri	https://opara.zih.tu-dresden.de/handle/123456789/1372
Public reference to this page dc.identifier.uri	https://doi.org/10.25532/OPARA-792
Publisher dc.publisher	Technische Universität Dresden
Licence dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 International	en
URI of the licence text dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
Specification of the discipline(s) dc.subject.classification	2
Specification of the discipline(s) dc.subject.classification	3
Title of the dataset dc.title	Measuring active fluctuations of the cortex in mitosis by embedding an AFM cantilever pyrimidal tip
Project title opara.project.title	Time irreversibility, entropy production and effective temperature are independently regulated in the actin cortex of living cells