Chaperones Skp and SurA dynamically expand unfolded OmpX and synergistically disassemble oligomeric aggregates
datacite.FundingReference.funderName | Deutsche Forschungsgemeinschaft | |
Contributing person | Schlierf, Michael (orcid: 0000-0002-6209-2364) | |
Contributing person | Hartmann, Andreas | |
Contributing person | Svirina, Anna | |
Contributing person | Krainer, Georg | |
Contributing person | Ma, Mai Quynh | |
Contributing person | Chamachi, Neharika | |
Contributing person | Schlierf, Michael, TU Dresden | |
datacite.description.TableOfContents | Each compressed file (.zip file) name corresponds to the measurement sample (the variant name, and if present, the chaperone name). After uncompressing the archives, various folders are generated: - the "Raw data files" folder contains the acquired photon stream files in .ht3 format. - For all the FRET measurements presented in the manuscript, a folder named "BIN files - IPT-0p05-0p05-N-40" is also provided. It contains the analysed burst files with a maximum interphoton time of 0.05 ms, minimum interphoton noise of 0.05 ms, and a minimum total number of 40 photons. All the other settings used for analysis are mentioned in the "Settings.txt"*. - In the folders of OmpX 1 149 measurements without chaperones i.e. "xxDeg_0nM_Skp_Trimer" or "xxDeg_0nM_SurA", the "for RASP analysis" folder contains the pre-processed file used for RASP analysis. - In the folders with OmpX+Chaperone measurements, the "FRET_OmpX_Skp_xnM" or "FRET_OmpX_SurA_xnM" are the files used for performing global chi-square minimization routine to obtain the thermodynamic parameters. | |
Documentation of the data | Resource Type: - the acquired photon stream files in .ht3 format. - the analysed burst files called as BIN files - the pre-processed file used for RASP analysis. - the files used for performing global chi-square minimization routine to obtain the thermodynamic parameters. Data Acquisition: single-molecule FRET and FCS experiments Data Processing: The raw data is presented in .ht3 files. Fluorescence bursts were identified and saved in .bin files. Methods: FRET and FCS | |
Description of the data | raw HT3 and Bin File data This collection contains all raw data for the study of the interaction of OmpX with chaperones SurA and Skp3. The data was collected on a custom-built confocal TCSPC instrument as described in Hartmann and Krainer et al. Molecules 2014 and Hartmann and Krainer et al. Analytical Chemistry 2015. | |
Type of the data | Dataset | |
Total size of the dataset | 33913857720 | |
Author | Schlierf, Michael | |
Upload date | 2022-02-01T16:49:54Z | |
Publication date | 2022-02-01T16:49:54Z | |
Publication date | 2026-06-05T13:13:43Z | |
Data of data creation | 2017-2022 | |
Publication date | 2022-02-01 | |
Abstract of the dataset | The packages contain the raw data (.ht3 and BIN files) obtained for all smFRET and FCS measurements mentioned in the manuscript titled "Chaperones Skp and SurA dynamically expand unfolded OmpX and synergistically disassemble oligomeric aggregates." The data were acquired using a custom-built setup combining confocal singe-molecule spectroscopy with time-correlated single-photon counting (TCSPC), pulsed interleaved excitation (PIE), and fluorescence anisotropy detection for multiparameter-flourescence detection (MFD). | |
Public reference to this page | https://opara.zih.tu-dresden.de/handle/123456789/2539 | |
Public reference to this page | https://doi.org/10.25532/OPARA-162 | |
dc.language | eng | |
Publisher | Technische Universität Dresden | |
Licence | Attribution 4.0 International | |
URI of the licence text | http://creativecommons.org/licenses/by/4.0/ | |
Specification of the discipline(s) | 3::32 | |
Specification of the discipline(s) | 2::21 | |
Specification of the discipline(s) | 2::21::201::201-01 | |
Specification of the discipline(s) | 2 | |
Title of the dataset | Chaperones Skp and SurA dynamically expand unfolded OmpX and synergistically disassemble oligomeric aggregates | |
Research instruments | custom-built confocal TCSPC instrument as described in Hartmann, Krainer et al. Analytical Chemistry 2015 | |
Underlying research object | Recombinantly expressed proteins from E. coli, in particular OmpX, SurA and Skp | |
Project abstract | In this project we studied the structural properties of OmpX in buffer and in complex with the chaperones SurA and Skp. We used single-molecule FRET, FCS, FLCS and nsFCS to determine the structural dynamics across many different time scales. This deposit carries the raw data and the first analysis steps, like burst identification. | |
Project title | OmpX_chaperone_thermodynamics_Schlierf_lab (OmpChaT) |
Files
Original bundle
- Name:
- OmpX_1_119__-_Labeled_with_Atto532_AbStar635P_-_in_6_M_GdmCl_-_no_LDAO.zip
- Size:
- 1.54 GB
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- Name:
- OmpX_1_119_-_Labeled_with_Atto532_AbStar635P_-_with_and_without_chaperones.zip
- Size:
- 1.11 GB
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- Description:
- Name:
- OmpX_1_149_-_Labeled_with_Atto532_AbStar635P_-_Aggregation_Measurements.zip
- Size:
- 3.32 GB
- Format:
- Description:
- Name:
- OmpX_1_149_-_Labeled_with_Atto532_AbStar635P__in_6_M_GdmCl_-_no_LDAO.zip
- Size:
- 851.75 MB
- Format:
- Description:
- Name:
- OmpX_1_149_-_Labeled_with_Atto532_AbStar635P_+_Skp-Temperature_range_measurements.zip
- Size:
- 9.71 GB
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- Description:
- Name:
- OmpX_1_149_-_Labeled_with_Atto532_AbStar635P_+_SurA-Temperature_range_measurements.zip
- Size:
- 10.75 GB
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- Name:
- OmpX_1_149_-_Labeled_with_Atto532_Atto647N.zip
- Size:
- 1.7 GB
- Format:
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- Name:
- OmpX_1_149_-_Refolded_-_Labeled_with_Atto532_AbStar635P_-_20mMTrisHCl_2mMEDTA_10mM_LDAO_pH_8.zip
- Size:
- 732.22 MB
- Format:
- Description:
- Name:
- OmpX_35_149_-_Labeled_with_Atto532_AbStar635P_-_in_6_M_GdmCl_-_no_LDAO.zip
- Size:
- 827.61 MB
- Format:
- Description:
- Name:
- OmpX_35_149_-_Labeled_with_Atto532_AbStar635P_-_with_and_without_chaperones.zip
- Size:
- 1.1 GB
- Format:
- Description:
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