TU Dresden Data Publications

Permanent URI for this collectionhttp://opara.zih.tu-dresden.de/handle/123456789/15

Data publications from research of Dresden University of Technology.

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Browsing TU Dresden Data Publications by Subject "2::21::201::201-03"

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  • ItemOpen Access
    Research Data supporting the publication "Robust cytoplasmic partitioning by solving a cytoskeletal instability”
    (Technische Universität Dresden, 2025-10-23) Rinaldin, Melissa; Kickuth, Alison; Xu, Yitong; Brugués, Jan
    This dataset contains fluorescence imaging raw data and Python and ImageJ analysis scripts that were produced during the study of microtubule asters in frog egg extract and zebrafish and fruit fly embryos. The fluorescence images and time sequences consist of (1) investigations of stability and patterning phenotypes by labelling microtubules, DNA, lipids, and mitochondria, (2) study of microtubule density and polymerisation speed with imaging of EB1 comets, and (3) study of microtubule turnover with tubulin speckles and FRAP. The Research Data are organised by Figure and Extended Data Figure numbers of the manuscript, with detailed descriptions provided in the publication.
  • ItemOpen Access
    Supplemental data to "A mechanical ratchet drives unilateral cytokinesis"
    (Technische Universität Dresden, 2025-10-01) Kickuth, Alison; Ursic, Urša; Staddon, Michael Feroli; Brugués, Jan
    This dataset presents raw imaging data, quantitative measurements, and data visualisation code from zebrafish embryo experiments investigating contractile band dynamics during early cell division. The study examines the mechanical processes that drive unilateral cytokinesis, focusing on cytoskeletal organization across the cell cycle. High-resolution time-lapse microscopy and quantitative image analysis were used to capture and assess the cellular behaviours.
  • ItemOpen Access
    Supplementary data for the publication "Cell size reduction distinctly scales spindle elongation and chromosome segregation in C. elegans"
    (Technische Universität Dresden, 2026-06-29) Okafornta, Chukwuebuka William
    How embryos adapt their internal cellular machinery to reductions in cell size during development remains a fundamental question in cell biology. Here, we use high-resolution lattice light-sheet fluorescence microscopy and automated image analysis to quantify lineage-resolved mitotic spindle and chromosome segregation dynamics from the 2- to 64-cell stages in Caenorhabditis elegans embryos. While spindle length scales with cell size across both wild-type and size-perturbed embryos, chromosome segregation dynamics remain largely invariant, suggesting that distinct mechanisms govern these mitotic processes. Combining femtosecond laser ablation with large-scale electron tomography, we find that mid-spindle microtubules mediate chromosome segregation dynamics and remain uncoupled from cell size across all stages of early development. In contrast, spindle elongation is driven by cortically anchored motor proteins and astral microtubules, rendering it sensitive to cell size. Incorporating these experimental results into an extended stoichiometric model for both the spindle and chromosomes, we find that allowing only cell size and microtubule catastrophe rates to vary reproduces spindle pole-to-pole dynamics across development. The same model also accounts for centrosome separation and pronuclear positioning in the one-cell C. elegans embryo, spindle-length scaling across nematode species spanning ~100 million years of divergence, and spindle rotation in human cells. Thus, a unified stoichiometric framework provides a predictive, mechanistic account of spindle and nuclear dynamics across scales and species.