Universität Leipzig

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  • ItemOpen Access
    Adipic acid concentrations from biotransformation by rec. Pseudomonas taiwanensis
    (Universität Leipzig, 2025-02-05) Franz, Alexander
    The dataset belongs to a publication in Green Chemistry from 2023 (DOI: 10.1039/d3gc01105d). In the publication a two-step process is demonstrated, in which phenolic compounds are electrochemically hydrogenated and further converted to adipic acid by a biotransformation of recombinant Pseudomonas taiwanensis. The dataset shows the data from Fig. 3c and 4c in the publication. The two tables show the adipic acid concentration over time in the 500 mL-bubble column reactors, in which the biocatalyst was fed with the substrate solution.
  • ItemOpen Access
    Dataset for "Targeted, receptor-mediated delivery of a masked ᴅ-amino acid cell penetrating peptide for cell-specific phototoxicity"
    (Universität Leipzig, 2025-11-06) List, Moritz; Jülke, Eva-Maria; Beck-Sickinger, Annette G.
    Photodynamic therapy is an innovative treatment option for cancer, but current approaches are limited by poor tumor selectivity and low uptake. Here, we introduce a novel concept for a targeted phototoxic peptide, in which a lysosomally activatable payload is delivered selectively by receptor-mediated endocytosis. For the payload, 6-carboxytetramethylrhodamine (TMR) was attached to an activatable cell penetrating peptide. The regular activity of the CPP was blocked by electrostatic interactions with a poly-glutamate sequence but could be restored through cleavage by the lysosomal protease cathepsin B both in vitro and in cells. The unmasked CPP can then bind to the negatively charged lysosomal membrane, and upon irradiation, TMR generates reactive oxygen species (ROS) that disrupt the integrity of the membrane. This leads to a release of lysosomal contents into the cytosol which subsequently induces cell death. To achieve targeted delivery, the activatable payload was conjugated to chemerin-9, a high-affinity ligand for the chemokine-like receptor 1 (CMKLR1), a G protein-coupled receptor overexpressed in various cancers. Through this receptor-targeted approach, the peptide accumulates in CMKLR1-expressing cells while the lysosomal activation completely prevented off-target toxicity. Notably, this strategy enables even a weak photosensitizer like TMR to achieve potent cytotoxicity through lysosomal targeting. This approach represents an advancement in improving the selectivity and efficacy of photodynamic therapy and holds promise for the development of novel cancer therapies. Furthermore, the concept opens possibilities for specific intracellular delivery of peptides or proteins.