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Other contributing persons, institutions or organisationsdfg - Funder
Person(s) who is (are) responsible for the content of the research dataReinhardt, Klaus - TU Dresden (ORCID: 0000-0003-4205-2370)
Person(s) who is (are) responsible for the content of the research dataMassino, Christian - TU Dresden (ORCID: 0000-0002-7316-9507)
AbstractData to: Seminal fluid, and sperm diluent, affect sperm metabolism in an insect: evidence from NAD(P)H and FAD autofluorescence lifetime imaging (SemFSpMetab) Sperm metabolism is fundamental to sperm motility and male fertility. Its measurement is still in its infancy and recommendations do not exist as to whether or how to standardize laboratory procedures. Here, using the sperm of an insect, the common bedbug, Cimex lectularius, we demonstrate that standardization of sperm metabolism is required with respect to the artificial sperm storage medium and a natural medium, the seminal fluid. We used fluorescence lifetime imaging microscopy (FLIM) in combination with time-correlated single-photon counting (TCSPC) to quantify sperm metabolism based on the fluorescent properties of autofluorescent coenzymes, NAD(P)H and FAD. Autofluorescence lifetimes (decay times) differ for the free and protein-bound state of the co-enzymes, and their relative contributions to the lifetime signal serve to characterize the metabolic state of cells. We found that artificial storage medium and seminal fluid separately, and additively, affected sperm metabolism. In a medium containing sugars and amino acids (Grace's Insect Medium), sperm showed increased glycolysis compared to a commonly used storage medium, phosphate-buffered saline (PBS). Adding seminal fluid to the sperm additionally increased oxidative phosphorylation, likely reflecting increased energy production of sperm during activation. Our study provides a protocol to measure sperm metabolism independently from motility, stresses that protocol standardizations for sperm measurements should be implemented and, for the first time, demonstrates that seminal fluid alters sperm metabolism. Equivalent protocol standardizations should be imposed on metabolic investigations of human sperm samples.
Applied methods and techniquesFluorescence Lifetime Imaging Microscopy (FLIM)
Counties, the data is referencingGERMANYde
Languageeng
Year or period of data production2021
Publication year2021
PublisherTechnische Universität Dresden
References on related materialsIsPartOf: 123456789/1968 (Handle)
Content of the research dataDataset: FLIM Measurements of sperm cells of bedbugs after various treatments
Holder of usage rightsTechnische Universität Dresden
Usage rights of the dataCC-BY-4.0
Discipline(s)Biologyde
Title of the datasetFLIM Measurements of bed bug sperm cells


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