| Other contributing persons, institutions or organisations | Müller-Reichert, Thomas - EZ_CFCI (ORCID: 0000-0003-0203-1436) - ProjectLeader | |
| Person(s) who is (are) responsible for the content of the research data | Kiewisz, Robert - EZ-CFCI (ORCID: 0000-0003-2733-4978) | |
| Description of further data processing | Reconstruction in IMOD, Modeling in ZIB Amira, Analysis in ASGA v0.35 | |
| Used research instruments or devices | FEI F30 | |
| Used research instruments or devices | Wohlwend HPF compact 03 | |
| Used research instruments or devices | Leica AFS II | |
| Used research instruments or devices | Leica ultramicrotome UC5 | |
| Abstract | This collection of datasets belongs to the eLife paper titled "Three-dimensional structure of kinetochore-fibers in human mitotic spindles": The collection includes all raw datasets of three HeLa Kyoto metaphase cells, that were acquired using electron tomography technique. This collection is also included all processed data as well as analysis as were shown in the publication. | |
| Applied methods and techniques | high-pressure freezing, freeze substitution, epond infiltration and embedding, serial sectioning, Electron tomography | |
| Table of contents | Metaphase_#1
├── Analysis_ASGA_v0.35 # Excel sheets containing all analyzed results obtained from ASGA v0.35
├── MT_Models # 3D spatial graph models of MTs in HeLa #1
│ └── T_0475_meta_7A-7C_spatialgraph_All_MTs.am
│ └── T_0475_meta_7A-7C_spatialgraph_ASGA_Analysis.am
│ └── T_0475_meta_7A-7C_spatialgraph_KMTs.am
│ └── T_0494_meta_10B-10E_spatialgraph_ASGA_Analysed.am
├── Surfaces # 3D models obtained from tomographic volume segmentation with Zib Amira
│ └── T_0475_meta_7A-7C_Centrosome.am
│ └── T_0475_meta_7A-7C_Chromosome.am
└── Tomogram_Stack # Tomographic volume saved in binary .am format
Metaphase_#2
├── Analysis_ASGA_v0.35 # Excel sheets containing all analyzed results obtained from ASGA v0.35
├── MT_Models # 3D spatial graph models of MTs in HeLa #2
│ └── T_0479_meta_15A-15C_spatialgraph_All_MTs.am
│ └── T_0479_meta_15A-15C_spatialgraph_ASGA_Analysis.am
│ └── T_0479_meta_15A-15C_spatialgraph_KMTs.am
│ └── T_0479_meta_15A-15C_spatialgraph_ASGA_Analysed.am
├── Surfaces # 3D models obtained from tomographic volume segmentation with Zib Amira
│ └── T_0479_meta_15A-15C_Centrosome.am
│ └── T_0479_meta_15A-15C_Chromosome.am
└──Tomogram_Stack # Tomographic volume saved in binary .am format
Metaphase_#3
├── Analysis_ASGA_v0.35 # Excel sheets containing all analyzed results obtained from ASGA v0.35
├── MT_Models # 3D spatial graph models of MTs in HeLa #3
│ └── T_0494_meta_10B-10E_spatialgraph_All_MTs.am
│ └── T_0494_meta_10B-10E_spatialgraph_ASGA_Analysis.am
│ └── T_0494_meta_10B-10E_spatialgraph_KMTs.am
│ └── T_0494_meta_10B-10E_spatialgraph_ASGA_Analysed.am
├── Surfaces # 3D models obtained from tomographic volume segmentation with Zib Amira
│ └── T_0494_meta_10B-10E_Centrosome.am
│ └── T_0494_meta_10B-10E_Chromosome.am
└──Tomogram_Stack # Tomographic volume saved in binary .am format | |
| Counties, the data is referencing | GERMANY | de |
| Regions the data is referencing | Saxony | |
| Language | eng | |
| Year or period of data production | 2021 | |
| Publication year | 2021 | |
| Publisher | Technische Universität Dresden | |
| Publisher | Robert Kiewisz | |
| References on related materials | IsPartOf: 123456789/1958 (Handle) | |
| Content of the research data | Text, Model: 3D tomographic model of HeLa cell obtained with 300kV TEM | |
| Holder of usage rights | Technische Universität Dresden | |
| Usage rights of the data | CC-BY-4.0 | |
| Software | Resource Production: IMOD 4.12.8 | |
| Software | Resource Production: Zib Amira 2021.05 | |
| Software | Resource Production: ASGA 0.35 | |
| Discipline(s) | Biology | de |
| Discipline(s) | Life Science | de |
| Title of the dataset | HeLa metaphase spindle tomographic data sets and analysis | |
