Show simple item record

Metadata

Other contributing persons, institutions or organisationsMüller-Reichert, Thomas - EZ_CFCI (ORCID: 0000-0003-0203-1436) - ProjectLeader
Person(s) who is (are) responsible for the content of the research dataKiewisz, Robert - EZ-CFCI (ORCID: 0000-0003-2733-4978)
Description of further data processingReconstruction in IMOD, Modeling in ZIB Amira, Analysis in ASGA v0.35
Used research instruments or devicesFEI F30
Used research instruments or devicesWohlwend HPF compact 03
Used research instruments or devicesLeica AFS II
Used research instruments or devicesLeica ultramicrotome UC5
AbstractThis collection of datasets belongs to the eLife paper titled "Three-dimensional structure of kinetochore-fibers in human mitotic spindles": The collection includes all raw datasets of three HeLa Kyoto metaphase cells, that were acquired using electron tomography technique. This collection is also included all processed data as well as analysis as were shown in the publication.
Applied methods and techniqueshigh-pressure freezing, freeze substitution, epond infiltration and embedding, serial sectioning, Electron tomography
Table of contentsMetaphase_#1 ├── Analysis_ASGA_v0.35 # Excel sheets containing all analyzed results obtained from ASGA v0.35 ├── MT_Models # 3D spatial graph models of MTs in HeLa #1 │ └── T_0475_meta_7A-7C_spatialgraph_All_MTs.am │ └── T_0475_meta_7A-7C_spatialgraph_ASGA_Analysis.am │ └── T_0475_meta_7A-7C_spatialgraph_KMTs.am │ └── T_0494_meta_10B-10E_spatialgraph_ASGA_Analysed.am ├── Surfaces # 3D models obtained from tomographic volume segmentation with Zib Amira │ └── T_0475_meta_7A-7C_Centrosome.am │ └── T_0475_meta_7A-7C_Chromosome.am └── Tomogram_Stack # Tomographic volume saved in binary .am format Metaphase_#2 ├── Analysis_ASGA_v0.35 # Excel sheets containing all analyzed results obtained from ASGA v0.35 ├── MT_Models # 3D spatial graph models of MTs in HeLa #2 │ └── T_0479_meta_15A-15C_spatialgraph_All_MTs.am │ └── T_0479_meta_15A-15C_spatialgraph_ASGA_Analysis.am │ └── T_0479_meta_15A-15C_spatialgraph_KMTs.am │ └── T_0479_meta_15A-15C_spatialgraph_ASGA_Analysed.am ├── Surfaces # 3D models obtained from tomographic volume segmentation with Zib Amira │ └── T_0479_meta_15A-15C_Centrosome.am │ └── T_0479_meta_15A-15C_Chromosome.am └──Tomogram_Stack # Tomographic volume saved in binary .am format Metaphase_#3 ├── Analysis_ASGA_v0.35 # Excel sheets containing all analyzed results obtained from ASGA v0.35 ├── MT_Models # 3D spatial graph models of MTs in HeLa #3 │ └── T_0494_meta_10B-10E_spatialgraph_All_MTs.am │ └── T_0494_meta_10B-10E_spatialgraph_ASGA_Analysis.am │ └── T_0494_meta_10B-10E_spatialgraph_KMTs.am │ └── T_0494_meta_10B-10E_spatialgraph_ASGA_Analysed.am ├── Surfaces # 3D models obtained from tomographic volume segmentation with Zib Amira │ └── T_0494_meta_10B-10E_Centrosome.am │ └── T_0494_meta_10B-10E_Chromosome.am └──Tomogram_Stack # Tomographic volume saved in binary .am format
Counties, the data is referencingGERMANYde
Regions the data is referencingSaxony
Languageeng
Year or period of data production2021
Publication year2021
PublisherTechnische Universität Dresden
PublisherRobert Kiewisz
References on related materialsIsPartOf: 123456789/1958 (Handle)
Content of the research dataText, Model: 3D tomographic model of HeLa cell obtained with 300kV TEM
Holder of usage rightsTechnische Universität Dresden
Usage rights of the dataCC-BY-4.0
SoftwareResource Production: IMOD 4.12.8
SoftwareResource Production: Zib Amira 2021.05
SoftwareResource Production: ASGA 0.35
Discipline(s)Biologyde
Discipline(s)Life Sciencede
Title of the datasetHeLa metaphase spindle tomographic data sets and analysis


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record