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HeLa metaphase spindle tomographic data sets and analysis
Metadaten
| Weitere mitwirkende Personen, Institutionen oder Unternehmen | Müller-Reichert, Thomas - EZ_CFCI (ORCID: 0000-0003-0203-1436) - ProjectLeader | |
| Für den Inhalt der Forschungsdaten verantwortliche Person(en) | Kiewisz, Robert - EZ-CFCI (ORCID: 0000-0003-2733-4978) | |
| Beschreibung der weiteren Datenverarbeitung | Reconstruction in IMOD, Modeling in ZIB Amira, Analysis in ASGA v0.35 | |
| Verwendete Forschungsinstrumente | FEI F30 | |
| Verwendete Forschungsinstrumente | Wohlwend HPF compact 03 | |
| Verwendete Forschungsinstrumente | Leica AFS II | |
| Verwendete Forschungsinstrumente | Leica ultramicrotome UC5 | |
| Kurzbeschreibung | This collection of datasets belongs to the eLife paper titled "Three-dimensional structure of kinetochore-fibers in human mitotic spindles": The collection includes all raw datasets of three HeLa Kyoto metaphase cells, that were acquired using electron tomography technique. This collection is also included all processed data as well as analysis as were shown in the publication. | |
| Angewendete Methoden oder Verfahren | high-pressure freezing, freeze substitution, epond infiltration and embedding, serial sectioning, Electron tomography | |
| Inhaltsverzeichnis | Metaphase_#1 ├── Analysis_ASGA_v0.35 # Excel sheets containing all analyzed results obtained from ASGA v0.35 ├── MT_Models # 3D spatial graph models of MTs in HeLa #1 │ └── T_0475_meta_7A-7C_spatialgraph_All_MTs.am │ └── T_0475_meta_7A-7C_spatialgraph_ASGA_Analysis.am │ └── T_0475_meta_7A-7C_spatialgraph_KMTs.am │ └── T_0494_meta_10B-10E_spatialgraph_ASGA_Analysed.am ├── Surfaces # 3D models obtained from tomographic volume segmentation with Zib Amira │ └── T_0475_meta_7A-7C_Centrosome.am │ └── T_0475_meta_7A-7C_Chromosome.am └── Tomogram_Stack # Tomographic volume saved in binary .am format Metaphase_#2 ├── Analysis_ASGA_v0.35 # Excel sheets containing all analyzed results obtained from ASGA v0.35 ├── MT_Models # 3D spatial graph models of MTs in HeLa #2 │ └── T_0479_meta_15A-15C_spatialgraph_All_MTs.am │ └── T_0479_meta_15A-15C_spatialgraph_ASGA_Analysis.am │ └── T_0479_meta_15A-15C_spatialgraph_KMTs.am │ └── T_0479_meta_15A-15C_spatialgraph_ASGA_Analysed.am ├── Surfaces # 3D models obtained from tomographic volume segmentation with Zib Amira │ └── T_0479_meta_15A-15C_Centrosome.am │ └── T_0479_meta_15A-15C_Chromosome.am └──Tomogram_Stack # Tomographic volume saved in binary .am format Metaphase_#3 ├── Analysis_ASGA_v0.35 # Excel sheets containing all analyzed results obtained from ASGA v0.35 ├── MT_Models # 3D spatial graph models of MTs in HeLa #3 │ └── T_0494_meta_10B-10E_spatialgraph_All_MTs.am │ └── T_0494_meta_10B-10E_spatialgraph_ASGA_Analysis.am │ └── T_0494_meta_10B-10E_spatialgraph_KMTs.am │ └── T_0494_meta_10B-10E_spatialgraph_ASGA_Analysed.am ├── Surfaces # 3D models obtained from tomographic volume segmentation with Zib Amira │ └── T_0494_meta_10B-10E_Centrosome.am │ └── T_0494_meta_10B-10E_Chromosome.am └──Tomogram_Stack # Tomographic volume saved in binary .am format | |
| Länder, auf die sich die Daten beziehen | GERMANY | de |
| Region(en) auf die sich die Daten beziehen | Saxony | |
| Sprache | eng | |
| Entstehungsjahr oder Entstehungszeitraum | 2021 | |
| Veröffentlichungsjahr | 2021 | |
| Herausgeber | Technische Universität Dresden | |
| Herausgeber | Robert Kiewisz | |
| Referenzen auf ergänzende Materialien | IsPartOf: 123456789/1958 (Handle) | |
| Inhalt der Forschungsdaten | Text, Model: 3D tomographic model of HeLa cell obtained with 300kV TEM | |
| Inhaber der Nutzungsrechte | Technische Universität Dresden | |
| Nutzungsrechte des Datensatzes | CC-BY-4.0 | |
| Eingesetzte Software | Resource Production: IMOD 4.12.8 | |
| Eingesetzte Software | Resource Production: Zib Amira 2021.05 | |
| Eingesetzte Software | Resource Production: ASGA 0.35 | |
| Angabe der Fachgebiete | Biology | de |
| Angabe der Fachgebiete | Life Science | de |
| Titel des Datensatzes | HeLa metaphase spindle tomographic data sets and analysis |