This dataset contains the raw sequences and images of 24 selected thin sections cut from 17 tooth samples, which were examined with advanced polarized light microscopy. Using a polarization camera with a 2x2 pattern of polarizers on the sensor and varying linearly polarized illumination states, depolarized light microscopy images were created by evaluating the degree of polarization (DOP) within thin sections. To generate the data, the thin sections were examined using a modified Leica DMRB microscope (Leica Microsystems GmbH, Wetzlar, Germany) with the complementary implementation of a FLIR polarization camera (FLIR Integrated Imaging Solutions Inc., Ludwigsburg, Germany). The pixel size of the processed images is 3.93 µm x 3.93 µm. Since multiple areas of interests were examined for several thin sections, 29 data sets are available. For each data set, the sequence of raw images of the polarization camera as well as processed DLM images including representations of the DOP and of the intensity are available. Note, that the DLM images must be flipped horizontally compared to the conventional polarized light microscopy images acquired with the same microscope.

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