TU Dresden Data Publications
Permanent URI for this collectionhttp://opara.zih.tu-dresden.de/handle/123456789/15
Data publications from research of Dresden University of Technology.
Browse
Browsing TU Dresden Data Publications by Subject "2::21"
Now showing 1 - 15 of 15
- Results Per Page
- Sort Options
Item Open Access 2P NAD(P)H/FAD FLIM Drosophila melanogaster tissues (FLIM_Dmel_tissues)(Technische Universität Dresden, 2019-09-03) Wetzker, CorneliaTwo-photon NAD(P)H/FAD fluorescence lifetime imaging data of intact Drosophila melanogaster tissues including salivary gland and fat body cells of third instar larvae, enterocytes, as well as sperm stored the male seminal vesicle and the female seminal receptacle.Item Open Access Bioinformatic comparative genomic analysis of Psp proteins in bacteria and archaea(Technische Universität Dresden, 2021-06-15) Wolf, Diana; Gumerov, Vadim; Popp, Philipp; Bewersdorf, Lisa; Mascher, Thorsten; Andrianova, Ekaterina; Wolf, Diana; Zhulin, IgorSupplemental material for 'Phyletic distribution and diversification of the Phage Shock Protein stress response system in bacteria and archaea'Item Open Access Carbon to nitrogen (C/N) ratios, δ15N [‰] and δ13C [‰] of peat core increments(Technische Universität Dresden, 2020-04-24) Zeh, LilliDetailed description on data acquisition can be found here: Zeh, L., Igel, T. M., Schellekens, J., Limpens, J., Bragazza, L., & Kalbitz, K. (2020). Vascular plants affect properties and decomposition of moss-dominated peat, particularly at elevated temperatures. Biogeosciences Discussions, 1-29.Item Open Access Carbon to nitrogen (C/N) ratios, δ15N [‰] and δ13C [‰] of shoot and root tissues from three peat forming plants(Technische Universität Dresden, 2020-04-24) Zeh, LilliDetailed description on data acquisition can be found here: Zeh, L., Igel, T. M., Schellekens, J., Limpens, J., Bragazza, L., & Kalbitz, K. (2020). Vascular plants affect properties and decomposition of moss-dominated peat, particularly at elevated temperatures. Biogeosciences Discussions, 1-29.Item Open Access Chaperones Skp and SurA dynamically expand unfolded OmpX and synergistically disassemble oligomeric aggregates(Technische Universität Dresden, 2022-02-01) Schlierf, MichaelThe packages contain the raw data (.ht3 and BIN files) obtained for all smFRET and FCS measurements mentioned in the manuscript titled "Chaperones Skp and SurA dynamically expand unfolded OmpX and synergistically disassemble oligomeric aggregates." The data were acquired using a custom-built setup combining confocal singe-molecule spectroscopy with time-correlated single-photon counting (TCSPC), pulsed interleaved excitation (PIE), and fluorescence anisotropy detection for multiparameter-flourescence detection (MFD).Item Open Access Dataset and figures for "Sex differences in bedbug nymphs, Cimex lectularius"(Technische Universität Dresden, 2019-09-02) Langer, Lucas; Klaus, Reinhardt; Christin, FroschauerWe show two characters that can be used to separate bedbug nymphs. Given the bedbugs increasing status as a global pest and as a research model, we discuss the economic and biological consequences of these findings.Item Open Access FLIM Measurements of bed bug sperm cells(Technische Universität Dresden, 2021-09-01) Reinhardt, Klaus; Massino, ChristianData to: Seminal fluid, and sperm diluent, affect sperm metabolism in an insect: evidence from NAD(P)H and FAD autofluorescence lifetime imaging (SemFSpMetab) Sperm metabolism is fundamental to sperm motility and male fertility. Its measurement is still in its infancy and recommendations do not exist as to whether or how to standardize laboratory procedures. Here, using the sperm of an insect, the common bedbug, Cimex lectularius, we demonstrate that standardization of sperm metabolism is required with respect to the artificial sperm storage medium and a natural medium, the seminal fluid. We used fluorescence lifetime imaging microscopy (FLIM) in combination with time-correlated single-photon counting (TCSPC) to quantify sperm metabolism based on the fluorescent properties of autofluorescent coenzymes, NAD(P)H and FAD. Autofluorescence lifetimes (decay times) differ for the free and protein-bound state of the co-enzymes, and their relative contributions to the lifetime signal serve to characterize the metabolic state of cells. We found that artificial storage medium and seminal fluid separately, and additively, affected sperm metabolism. In a medium containing sugars and amino acids (Grace's Insect Medium), sperm showed increased glycolysis compared to a commonly used storage medium, phosphate-buffered saline (PBS). Adding seminal fluid to the sperm additionally increased oxidative phosphorylation, likely reflecting increased energy production of sperm during activation. Our study provides a protocol to measure sperm metabolism independently from motility, stresses that protocol standardizations for sperm measurements should be implemented and, for the first time, demonstrates that seminal fluid alters sperm metabolism. Equivalent protocol standardizations should be imposed on metabolic investigations of human sperm samples.Item Open Access HeLa metaphase spindle tomographic data sets and analysis(Technische Universität Dresden, 2021-09-02) Kiewisz, RobertThis collection of datasets belongs to the eLife paper titled "Three-dimensional structure of kinetochore-fibers in human mitotic spindles": The collection includes all raw datasets of three HeLa Kyoto metaphase cells, that were acquired using electron tomography technique. This collection is also included all processed data as well as analysis as were shown in the publication.Item Open Access HeLa metaphase spindle tomographic data sets and analysis for z-expansion data(Technische Universität Dresden, 2022-05-09) Kiewisz, Robert3D tomographic model of HeLa cell obtained with 300kV TEMItem Open Access MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles(Technische Universität Dresden, 2022-11-21) Kiewisz, RobertThis collection contains electron tomography data of HeLa cells depleted with MCRS1 protein. Datasets contain, a collection of spatial graphs.Item Open Access Pyrolysis data to reveal molecular composition of peat and peat forming plants(Technische Universität Dresden, 2020-04-23) Zeh, LilliPyrolysis gas chromatography/mass spectrometry data of peat and peat forming plants. Pyrolysis product identification and peak integration in pyrograms were performed with Masslab. The relative proportion of each product was expressed as a percentage of the total quantified peak area in one sample (TIC: 100 %). Detailed data description can be found here: Zeh, L., Igel, T. M., Schellekens, J., Limpens, J., Bragazza, L., & Kalbitz, K. (2020). Vascular plants affect properties and decomposition of moss-dominated peat, particularly at elevated temperatures. Biogeosciences Discussions, 1-29.Item Open Access Sperm viability varies with buffer and genotype in Drosophila melanogaster(Technische Universität Dresden, 2020-05-25) Reinhardt, Klaus; Henke, Anna-Lena; Guo, RuijianVariation in sperm viability in Drosophila melanogaster with buffer and genotypeItem Open Access Supplemental data for the publication "Male meiotic spindle features that efficiently segregate paired and lagging chromosomes" in eLife(Technische Universität Dresden, 2020-01-20) Fabig, GunarThis is the collection of all fully reconstructed spindles used throughout the publication "Male meiotic spindle features that efficiently segregate paired and lagging chromosomes".Item Open Access Tomographic models of male meiotic spindles(Technische Universität Dresden, 2019-08-21) Fabig, Gunar3D Model of microtubules and chromosomes from electron tomograms of male meiotic spindlesItem Open Access Ultrastructure of the nebenkern and male testes of H. membranacea(Technische Universität Dresden, 2023-06-07) Fabig, GunarSupplemental data for the PLoS One publication. Contains raw data (light microscopy and TEM) and electron tomograms with segmentations