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DNA ruler measurement for accuracy and precision of multiwell plate smFRET experiments
Metadata
| Other contributing persons, institutions or organisations | Hartmann, Andreas - TU Dresden (ORCID: 0000-0002-2213-2763) - WorkPackageLeader | |
| Other contributing persons, institutions or organisations | Schlierf, Michael - ProjectLeader | |
| Person(s) who is (are) responsible for the content of the research data | Schlierf, Michael | |
| Type of data acquisition | Observation: smFRET experiments | |
| Used research instruments or devices | custom-built multiwell plate, multiparameter smFRET microscope | |
| Research objects | Other: dsDNA | |
| Abstract | HT3 data and analysed *.bin files for 96 measurement repeats A01 to H12 in a 96-well plate, multiparameter detection smFRET experiment. Data can be analysed and visualised using autoFRET package at https://github.com/SchlierfLAB/autoFRET | |
| Applied methods and techniques | time-correlated single photon counting for single-molecule FRET spectroscopy | |
| Additional descriptive information to understand the data | software package for data curation, data analysis, and data visualisation can be found here: https://github.com/SchlierfLAB/autoFRET | |
| Additional keywords | smFRET; multiwell experiments; DNA ruler | |
| Year or period of data production | 2021-2023 | |
| Publication year | 2023 | |
| Publisher | Technische Universität Dresden | |
| References on related materials | IsPartOf: 123456789/5807 (Handle) | |
| Content of the research data | Dataset: Photon streams of 96-well (A01 to H12) smFRET data on dsDNA with donor-acceptor pairs at 9 and 21 bp distance. Instrument response functions (IRF) of the data set. | |
| Holder of usage rights | Technische Universität Dresden | |
| Holder of usage rights | Schlierf Lab | |
| Usage rights of the data | CC-BY-NC-4.0 | |
| Software | Other: autoFRET | |
| Additional precise description of discipline | Single-Molecule Biophysics | |
| Discipline(s) | Biochemistry | de |
| Discipline(s) | Physics | de |
| Discipline(s) | Other | de |
| Title of the dataset | DNA ruler measurement for accuracy and precision of multiwell plate smFRET experiments |
Files in this item
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smFRET data for multiwell plate measurements [5]
raw (ht3) and pre-analysed (bin) data of smFRET measurements for high-content, multiwell plate smFRET experiments.