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Depolarized light microscopy (DLM) images of occlusal caries lesions
Metadaten
| Weitere mitwirkende Personen, Institutionen oder Unternehmen | Golde, Jonas - Arbeitsbereich Medizinische Physik und Biomedizinische Technik - ProjectLeader | |
| Für den Inhalt der Forschungsdaten verantwortliche Person(en) | Grundmann, Julia | |
| Verwendete Forschungsinstrumente | Leica DMRB polarizing microscope (Leica Microsystems GmbH, Wetzlar, Germany); FLIR polarization camera (FLIR Integrated Imaging Solutions Inc., Ludwigsburg, Germany) | |
| Zugrundeliegende Forschungsobjekte | Tissue: thin sections of teeth with carious lesions | |
| Kurzbeschreibung | This dataset contains the images of 24 selected thin sections examined with depolarized light microscopy. Depolarized light microscopy is an advancement of conventional polarized light microscopy evaluating the degree of polarization (DOP) within thin sections. To generate the data, the thin sections were examined with the Leica DMRB polarizing microscope (Leica Microsystems GmbH, Wetzlar, Germany) with complementary implementation of a FLIR polarization camera (FLIR Integrated Imaging Solutions Inc., Ludwigsburg, Germany). The pixel size of the processed images is 3.93 µm x 3.93 µm. Since multiple areas of interests were examined within some thin sections, 29 data sets are available. For each data set, the raw data as well as a processed DLM image are available. Note that the processed DLM images must be flipped horizontally once in order to compare them with further optical imaging techniques from the same perspective. | |
| Angewendete Methoden oder Verfahren | As discussed elsewhere in detail, a polarization camera with 2x2 pattern of adjacent polarizers on the sensor was utilized for data acquisition. Each sequence consists of such raw images, which were captured during continuous rotation of a polarizer in the illumination path of the modified microscope. For creating the processed images, Stokes parameters and the degree of linear polarization (DOLP) were calculated from the raw images. By applying a maximum projection on the sequence of DOLP images, the degree of polarization (DOP) was extracted. | |
| Weitere Schlagwörter | microscopy, histology, depolarization | |
| Sprache | deu | |
| Entstehungsjahr oder Entstehungszeitraum | 2022 | |
| Veröffentlichungsjahr | 2023 | |
| Herausgeber | Technische Universität Dresden | |
| Referenzen auf ergänzende Materialien | IsPartOf: 123456789/5857 (Handle) | |
| Inhalt der Forschungsdaten | Image, Dataset: microscopy images of carious teeth | |
| Inhaber der Nutzungsrechte | TU Dresden | |
| Nutzungsrechte des Datensatzes | CC-BY-4.0 | |
| Eingesetzte Software | Resource Production: LabView | |
| Angabe der Fachgebiete | Engineering | de |
| Angabe der Fachgebiete | Medicine | de |
| Angabe der Fachgebiete | Physics | de |
| Titel des Datensatzes | Depolarized light microscopy (DLM) images of occlusal caries lesions |
Dateien zu dieser Ressource
Die Datenpakete erscheinen in:
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depolarized light microscopy images [1]
This dataset contains the raw sequences and images of 24 selected thin sections cut from 17 tooth samples, which were examined with advanced polarized light microscopy. Using a polarization camera with a 2x2 pattern of polarizers on the sensor and varying linearly polarized illumination states, depolarized light microscopy images were created by evaluating the degree of polarization (DOP) within thin sections. To generate the data, the thin sections were examined using a modified Leica DMRB microscope (Leica Microsystems GmbH, Wetzlar, Germany) with the complementary implementation of a FLIR polarization camera (FLIR Integrated Imaging Solutions Inc., Ludwigsburg, Germany). The pixel size of the processed images is 3.93 µm x 3.93 µm. Since multiple areas of interests were examined for several thin sections, 29 data sets are available. For each data set, the sequence of raw images of the polarization camera as well as processed DLM images including representations of the DOP and of the intensity are available. Note, that the DLM images must be flipped horizontally compared to the conventional polarized light microscopy images acquired with the same microscope.