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Chaperones Skp and SurA dynamically expand unfolded OmpX and synergistically disassemble oligomeric aggregates
Metadaten
| Weitere mitwirkende Personen, Institutionen oder Unternehmen | Chamachi, Neharika - TU Dresden (ORCID: 0000-0002-4657-9092) - Researcher | |
| Weitere mitwirkende Personen, Institutionen oder Unternehmen | Hartmann, Andreas - TU Dresden (ORCID: 0000-0002-2213-2763) - Researcher | |
| Weitere mitwirkende Personen, Institutionen oder Unternehmen | Krainer, Georg - TU Dresden (ORCID: 0000-0002-9626-7636) - Researcher | |
| Weitere mitwirkende Personen, Institutionen oder Unternehmen | Svirina, Anna - TU Dresden - Researcher | |
| Weitere mitwirkende Personen, Institutionen oder Unternehmen | Ma, Mai Quynh - TU Dresden - Researcher | |
| Für den Inhalt der Forschungsdaten verantwortliche Person(en) | Schlierf, Michael - TU Dresden (ORCID: 0000-0002-6209-2364) | |
| Beschreibung der weiteren Datenverarbeitung | The raw data is presented in .ht3 files. Fluorescence bursts were identified and saved in .bin files. | |
| Art der Erhebung der Daten | Experiment: single-molecule FRET and FCS experiments | |
| Verwendete Forschungsinstrumente | custom-built confocal TCSPC instrument as described in Hartmann, Krainer et al. Analytical Chemistry 2015 | |
| Zugrundeliegende Forschungsobjekte | Other: Recombinantly expressed proteins from E. coli, in particular OmpX, SurA and Skp | |
| Kurzbeschreibung | The packages contain the raw data (.ht3 and BIN files) obtained for all smFRET and FCS measurements mentioned in the manuscript titled "Chaperones Skp and SurA dynamically expand unfolded OmpX and synergistically disassemble oligomeric aggregates." The data were acquired using a custom-built setup combining confocal singe-molecule spectroscopy with time-correlated single-photon counting (TCSPC), pulsed interleaved excitation (PIE), and fluorescence anisotropy detection for multiparameter-flourescence detection (MFD). | |
| Angewendete Methoden oder Verfahren | FRET and FCS | |
| Inhaltsverzeichnis | Each compressed file (.zip file) name corresponds to the measurement sample (the variant name, and if present, the chaperone name). After uncompressing the archives, various folders are generated: - the "Raw data files" folder contains the acquired photon stream files in .ht3 format. - For all the FRET measurements presented in the manuscript, a folder named "BIN files - IPT-0p05-0p05-N-40" is also provided. It contains the analysed burst files with a maximum interphoton time of 0.05 ms, minimum interphoton noise of 0.05 ms, and a minimum total number of 40 photons. All the other settings used for analysis are mentioned in the "Settings.txt"*. - In the folders of OmpX 1 149 measurements without chaperones i.e. "xxDeg_0nM_Skp_Trimer" or "xxDeg_0nM_SurA", the "for RASP analysis" folder contains the pre-processed file used for RASP analysis. - In the folders with OmpX+Chaperone measurements, the "FRET_OmpX_Skp_xnM" or "FRET_OmpX_SurA_xnM" are the files used for performing global chi-square minimization routine to obtain the thermodynamic parameters. | |
| Weitere Schlagwörter | outer membrane protein biogenesis | |
| Weitere Schlagwörter | single-molecule FRET | |
| Weitere Schlagwörter | FCS | |
| Weitere Schlagwörter | protein folding | |
| Weitere Schlagwörter | disaggregation | |
| Weitere Schlagwörter | thermodynamics | |
| Weitere Schlagwörter | nanosecond, microsecond and millisecond dynamics | |
| Sprache | eng | |
| Entstehungsjahr oder Entstehungszeitraum | 2017-2022 | |
| Veröffentlichungsjahr | 2022 | |
| Herausgeber | Technische Universität Dresden | |
| Referenzen auf ergänzende Materialien | IsPartOf: 123456789/2042 (Handle) | |
| Inhalt der Forschungsdaten | Dataset: - the acquired photon stream files in .ht3 format. - the analysed burst files called as BIN files - the pre-processed file used for RASP analysis. - the files used for performing global chi-square minimization routine to obtain the thermodynamic parameters. | |
| Inhaber der Nutzungsrechte | Michael Schlierf, TU Dresden | |
| Nutzungsrechte des Datensatzes | CC-BY-4.0 | |
| Angabe der Fachgebiete | Biochemistry | de |
| Angabe der Fachgebiete | Biology | de |
| Angabe der Fachgebiete | Life Science | de |
| Angabe der Fachgebiete | Physics | de |
| Titel des Datensatzes | Chaperones Skp and SurA dynamically expand unfolded OmpX and synergistically disassemble oligomeric aggregates |
Dateien zu dieser Ressource
Die Datenpakete erscheinen in:
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raw HT3 and Bin File data [1]
This collection contains all raw data for the study of the interaction of OmpX with chaperones SurA and Skp3. The data was collected on a custom-built confocal TCSPC instrument as described in Hartmann and Krainer et al. Molecules 2014 and Hartmann and Krainer et al. Analytical Chemistry 2015.